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Berenil acts as a poison of eukaryotic topoisomerase II
Author(s) -
Portugal José
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00363-7
Subject(s) - topoisomerase , dna , kinetoplast , dna supercoil , chemistry , biochemistry , catenation , enzyme , biophysics , biology , dna replication
The ability of the anti‐trypanosomal drug berenil to interfere with the activities of eukaryotic type II topoisomerases is evaluated using two different types of reactions catalysed by the enzyme: the relaxation of naturally supercoiled DNA and the decatenation of kinetoplast DNA (kDNA). The results indicate that berenil acts as an inhibitor of the catalytic activity, but the inhibiting ability appears to be smaller than for other minor‐groove binding ligands.