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Serum immunoglobulins interact with oligonucleotides
Author(s) -
Rykova E.Yu.,
Pautova L.V.,
Yakubov L.A.,
Karamyshev V.N.,
Vlassov V.V.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00360-2
Subject(s) - oligonucleotide , myoglobin , chemistry , antibody , albumin , biochemistry , microbiology and biotechnology , serum albumin , monoclonal antibody , dna , biology , immunology
The interaction of reactive derivatives of oligonucleotides bearing a 4‐[( N ‐2‐chloroethyl‐ N ‐methyl)amino]benzylamin residue at the 5′‐terminal phosphate with serum blood proteins has been investigated. It was found that the compounds react with serum albumin and immunoglobulins M and G, the reactivity increasing in the order: albumin < IgG < IgM. The reactions with immunoglobulins were inhibited in the presence of different oligonucleotides, DNA and heparin, suggesting the oligonucleotide binding to some cationic region of the proteins. Myoglobin inhibited the interaction of oligonucleotide derivatives with myoglobin‐specific monoclonal antibodies which indicates that the derivatives interact with the proteins within or near the antigen binding site.