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Recognition of UUN codons by two leucine tRNA species from Escherichia coli
Author(s) -
Takai Kazuyuki,
Horie Nobuyuki,
Yamaizumi Ziro,
Nishimura Susumu,
Miyazawa Tatsuo,
Yokoyama Shigeyuki
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00354-8
Subject(s) - transfer rna , wobble base pair , escherichia coli , biology , ef tu , leucine , biochemistry , nucleotide , genetic code , protein biosynthesis , aminoacyl trna , amino acid , rna , gene
Codon recognition by Escherichia coli tRNA Leu 4 and tRNA Leu 5 was investigated by analysis of the competition between two aminoacyl‐tRNA species in an in vitro protein synthesis. Both tRNA species strictly obey the wobble rule when they are in competition with other tRNA species. This is probably due to the post‐transcriptional modifications at the first position of the anticodon of these tRNA Leu species, supporting the proposal that the conformational rigidity of post‐transcriptionally modified pyrimidine nucleotides guarantees the correct codon recognition.