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Intracellular dynamics of ricin followed by fluorescence microscopy on living cells reveals a rapid accumulation of the dimeric toxin in the Golgi apparatus
Author(s) -
Lendaro Eugenio,
Ippoliti Rodolfo,
Bellelli Andrea,
Brunori Maurizio,
Evangelista Valtere,
Guidarini Dante,
Benedetti Pier Alberto
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00255-x
Subject(s) - ricin , golgi apparatus , lectin , fluorescence microscope , intracellular , internalization , microbiology and biotechnology , endosome , immunotoxin , toxin , chemistry , biophysics , fluorescein , fluorescence , biochemistry , biology , receptor , cell , in vitro , physics , quantum mechanics , cytotoxicity
The intracellular dynamics of fluorescent conjugates of the toxic lectin ricin was followed by video fluorescence microscopy on living CHO cells, demonstrating that the ricin heterodimer and its isolated B chain, after binding to the plasma membrane receptors, migrate to and accumulate in the Golgi apparatus following internalization. A ricin derivative labelled with fluorescein on the A chain and rhodamine on the B chain did not display significant splitting of the A‐B heterodimer during translocation of the toxin to the Golgi; this novel finding provides support for the hypothesis that further processing of ricin takes place in this cellular compartment.