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Coexpression of type I and type II IL‐1 receptors in the murine T helper 2 cell line D10N
Author(s) -
Brigelius-Flohé R.,
McCarthy J.E.G.,
Resch K.,
Szamel M.,
Martin M.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81791-w
Subject(s) - receptor , microbiology and biotechnology , biology , interleukin 1 receptor, type i , interleukin 21 receptor , cell culture , common gamma chain , interleukin 4 receptor , interleukin 1 receptor , immunology , interleukin , cytokine , biochemistry , genetics
IL‐1 receptor heterogeneity in murine lymphocytes was investigated by cross‐linking to [ 125 I]IL‐1α and competition with IL‐1 receptor antagonist, and the molecular identity of the IL‐1 receptors was identified with PCR using primers specific for type I and type II IL‐1 receptors. The thymoma cell line EI4 6.1 exhibits exclusively the 80 kDa receptors which proved to be the type I receptor according to PCR analysis. In the pre‐B cell line 70Z/3, predominantly a 60 kDa type II receptor but also a trace of type I receptor can be identified by PCR. The Th2 cell line D10N expresses both types of IL‐1 receptors in equivalent amounts according to cross‐linking experiments and PCR. The proliferative response of D10N cells to IL‐1 is inhibited by IL‐1 ra which according to cross‐linking affects the binding to the type I receptor only. It is concluded that coexpression of both types of IL‐1 receptors might be a characteristic of murine Th2 cells and that their growth‐dependence on IL‐1 is mediated by the type I receptor.