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Rab effector domain peptides stimulate the release of neurotransmitter from cell cultured synapses
Author(s) -
Richmond Janet,
Haydon Philip G.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81775-u
Subject(s) - rab , neurotransmission , effector , microinjection , gtp' , neurotransmitter , microbiology and biotechnology , synapse , synapsin , synaptic vesicle , biology , chemistry , gtpase , biochemistry , biophysics , neuroscience , receptor , vesicle , membrane , enzyme
The involvement of the small GTP‐binding protein rab3A in synaptic transmission was tested by presynaptic microinjection of guanine nucleotides and peptides corresponding to the effector domain of rab3A. When GTPγS injection was paired with presynaptic action potentials, the frequency of MIPSCs was increased and the evoked synaptic current was reduced in magnitude. To more specifically manipulate rab proteins, peptides were microinjected. Injection of the peptide rab3AL(33‐48) or rab3(29‐48) stimulated an increase in the frequency of MIPSC with little effect on action potential evoked synaptic transmission supporting a role for rab proteins in regulating synaptic transmission.