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Expression of hepatic calcium‐binding protein regucalcin mRNA is mediated through Ca 2+ /calmodulin in rat liver
Author(s) -
Shimokawa Noriaki,
Yamaguchi Masayoshi
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81740-q
Subject(s) - calmodulin , trifluoperazine , endocrinology , medicine , messenger rna , protein kinase c , calcium , calcium binding protein , intraperitoneal injection , protein kinase a , northern blot , biology , signal transduction , gene expression , chemistry , microbiology and biotechnology , kinase , biochemistry , gene
The effect of signal transduction‐related factors was investigated to clarify the expression mechanism for mRNA of the hepatic Ca 2+ ‐binding protein regucalcin in the liver of rats. The change of regucalcin mRNA levels was analyzed by Northern blotting using liver regucalcin cDNA (0.6 kb). A single intraperitoneal administration of calcium chloride (15 mg Ca 2+ : 0.374 mmol/100 g body weight) to rats induced a remarkable increase of regucalcin mRNA in liver; the level was about 170% of controls at 30 min after administration. This increase was completely inhibited by simultaneous administration of trifluoperazine (5.0 mg/100 g), an antagonist of calmodulin. On the other hand, a single intraperitoneal administration of phorbol ester or dibutyryl cAMP (10–1,000 μg/100 g) did not cause a significant alteration of hepatic regucalcin mRNA levels. Also, administration of zinc, copper and cadmium (0.374 mmol of metal ion/100g) did not have an appreciable effect on hepatic regucalcin mRNA levels. These findings demonstrate that the expression of hepatic regucalcin mRNA is mediated through Ca 2+ /calmodulin.