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Expression of mRNAs encoding the α1 and the β1 subunits of Na + ,K + ‐ATPase in human lymphocytes activated with phytohaemagglutinine
Author(s) -
Vereninov A.A.,
Marakhova I.I.,
Osipov V.V.,
Toropova F.V.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81732-f
Subject(s) - cycloheximide , microbiology and biotechnology , messenger rna , atpase , protein subunit , chemistry , diaphragm pump , beta (programming language) , biology , enzyme , biochemistry , protein biosynthesis , gene , physics , piezoelectricity , acoustics , computer science , programming language
Increase in Na + ,K + ‐ATPase mRNAs was detected in activated lymphocytes by the RT‐PCR method. α1 subunit mRNA gradualy increased with time and by 36 h was 2.4 times higher than at the start. Increase in the β1 mRNA was transient reaching a maximum in the 8 h probe and declining to the initial level in the 24 and 36 h probes. The elevation of Na + ,K − ‐ATPase mRNAs does not underlie a cycloheximide‐inhibited increase in cation pumping peculiar to the prereplicative period as can be judged from the fact that Act D fails to eliminate PHA‐induced enhancement of pump fluxes.