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Role of a serum phospholipase A 1 in the phosphatidylserine‐induced T cell inhibition
Author(s) -
Bellini Fabrizio,
Bruni Alessandro
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81724-e
Subject(s) - phosphatidylethanolamine , phosphatidylserine , phosphatidylcholine , phosphatidylinositol , biochemistry , phospholipase , chemistry , fatty acid , phospholipase a2 , inhibitory postsynaptic potential , phospholipase c , phospholipid , enzyme , biology , signal transduction , membrane , neuroscience
We have previously shown that unsaturated phosphatidylserines inhibit mitogen‐induced T cell activation. We now report that the inhibitory action requires a protein present in bovine and human serum. Partial purification and phospholipase assay show that this protein has phospholipase A activity on phosphatidylserine but not phosphatidylethanolamine, phosphatidylcholine and phosphatidylinositol. In short incubations (1–3 h) 2‐acyl lysophosphatidylserine is produced but in longer incubations the cis ‐unsaturated fatty acid is also released. Experiments on peripheral blood mononuclear cells indicate that the unsaturated fatty acid becomes the main responsible for the PS‐induced inhibition and that 2‐acyl lysophosphatidylserine enhances the inhibitory effect of fatty acid.