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Molecular cloning of crustacean putative molt‐inhibiting hormone (MIH) precursor
Author(s) -
Klein Jörg M.,
Mangerich Sigrid,
de Kleijn Dominique P.V.,
Keller Rainer,
Weidemann Wolfgang M.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81699-z
Subject(s) - cloning (programming) , molecular cloning , crustacean , biology , hormone , chemistry , biochemistry , gene , peptide sequence , zoology , computer science , programming language
A cDNA encoding the complete precursor of the putative molt‐inhibiting hormone (MIH) of the shore crab, Carcinus maenas , was isolated and sequenced. The precursor consists of a putative 35 amino acid signal peptide and the 78 amino acid mature MIH. The deduced MIH amino acid sequence is in complete agreement with the sequence previously determined by Edman degradation. In situ hybridization revealed MIH‐expression in a subpopulation of large neurosecretory perikarya of the medulla terminalis X‐organ in the eyestalk.