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Expression of non‐hepatic‐type S ‐adenosylmethionine synthetase isozyme in rat hepatomas induced by 3′‐methyl‐4‐dimethylaminoazobenzene
Author(s) -
Hirokawa Saburo,
Kobayashi Yuki,
Sugiyama Toshihiro,
Terashima Hiromichi,
Wada Kenji,
Tsukada Kinji
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81682-p
Subject(s) - isozyme , enzyme , carcinogen , carcinogenesis , biology , messenger rna , gene , biochemistry , hepatocellular carcinoma , gene expression , microbiology and biotechnology , chemistry , cancer research
It is known that a high incidence of hepatocellular carcinoma in rat liver can be induced by such azo dye carcinogens as 3′‐methyl‐4‐dimethylaminoazobenzene (3′‐MeDAB). Mammalian S ‐adenosylmethionine (AdoMet) synthetase exists as two isozymes, non‐hepatic‐type and liver‐type enzymes, which are the products of two different genes. We have examined the expression of two AdoMet synthetase isozyme proteins and mRNAs in rat hepatomas induced by 3′‐Me‐DAB. The levels of non‐hepatic‐type enzyme protein and mRNA are clearly induced by 3′‐Me‐DAB feeding. On the other hand, the levels of liver‐type enzyme protein and mRNA are nearly the same or slightly decreased during hepatocarcinogenesis. These results indicate that the expression of the non‐hepatic‐type isozyme gene is obviously influenced with the progression of carcinogenesis and that the non‐hepatic‐type isozyme is useful as a oncodevelopmental marker in the liver.