Premium
Studies on the ability of minor groove binders to induce supercoiling in DNA
Author(s) -
Störl K.,
Burckhardt G.,
Lown J.W.,
Zimmer Ch.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81678-s
Subject(s) - minor groove , dna supercoil , dna , groove (engineering) , biophysics , chemistry , materials science , biology , biochemistry , dna replication , metallurgy
The effect of various non‐intercalating minor groove binders on closed circular DNA in the presence of topoisomerase I has been studied by means of agarose gel electrophoresis. Analogues of the netropsin series (lexitropsins) and SN‐6999 can effectively produce positive supercoils, as indicated by analysis of the topoisomers in the presence of chloroquine and the evaluated linking number changes. Analogues of the distamycin series are less effective, and bisquaternary ammonium heterocycles, as well as DAPI and pentamidine, were found to be ineffective ligands. The large differences observed in the ability of minor groove binders to induce positive supercoils are discussed.