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Distribution of non‐class‐III β‐tubulin isoforms in neuronal and non‐neuronal cells
Author(s) -
Linhartová Irena,
Dráberová Eduarda,
Viklický Vladimír,
Dráber Pavel
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81662-j
Subject(s) - gene isoform , tubulin , biology , microtubule , isoelectric focusing , microbiology and biotechnology , hela , monoclonal antibody , biochemistry , cell culture , in vitro , antibody , enzyme , genetics , gene
β‐Tubulin isoforms in brain tissues and in cell lines were analyzed by high‐resolution isoelectric focusing in combination with monoclonal antibodies. Post‐translational modifications of brain non‐class‐III β‐tubulin isoforms were found in phylogenetically distant species ranging from pig to carp. Less extensive modifications were also observed in Neuro‐2a, HeLa and 3T3 cells, where most acidic isoforms were glutamylated, while the basic, most abundant isoforms were not. The data suggest post‐translational modification of non‐class‐III β‐tubulin isoforms in neuronal as well as in non‐neuronal cells. Such modification might modulate interaction of tubulin isoforms with microtubule‐associated proteins.