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Chemical crosslinking studies of the isolated light‐harvesting B800‐850 complex of Chromatium minutissimum
Author(s) -
Moskalenko Andrey A.,
Toropygina Olga A.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81650-o
Subject(s) - propionate , chromatium , chemistry , bacteriochlorophyll , light harvesting complex , photochemistry , biophysics , biochemistry , photosynthesis , biology , photosystem ii
The spatial relationship of polypeptides comprising the light‐harvesting B800‐850 complex of Chromatium minutissimum has been studied by means of chemical crosslinking of the isolated complex with cleavable, 1.2 nm‐long dithiobis(succinimidyl propionate). The samples were analyzed by different types of electrophoresis and spectrophotometrically. No difference was shown between crosslinking of the B800‐850 complex either solubilized or incorporated into proteoliposomes. It was found that two main polypeptides form only one type of heterodimer. The crosslinked complex was more thermostable. This crosslinkage restricted the conformational transitions causing the shift of the long wavelength band in the near infrared region. A structure of the complex is discussed.