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A pH induced two‐dimensional crystal of membrane‐bound Na + ,K + ‐ATPase of dog kidney
Author(s) -
Tahara Yoshikazu,
Ohnishi Shun-ichi,
Fujiyoshi Yoshinori,
Kimura Yoshiaki,
Hayashi Yutaro
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81648-j
Subject(s) - crystallization , crystallography , electron microscope , membrane , chemistry , crystal (programming language) , crystal structure , electron crystallography , resolution (logic) , microscopy , sodium , analytical chemistry (journal) , diffraction , electron diffraction , chromatography , biochemistry , physics , optics , artificial intelligence , computer science , programming language , organic chemistry
Two‐dimensional crystals of membrane‐bound Na + ,K + ‐ATPase were formed in acidic media and their qualities were investigated by electron cryo‐microscopy as well as by conventional electron microscopy. At pH 4.8 in sodium citrate buffer, the best crystallization condition, more than 80% of membranes formed crystals. The high ratio allowed high‐resolution images to be taken by electron cryo‐microscopy. Image processing revealed that they had unique lattice constants ( a = 108.7 Å, b = 66.2 Å, γ = 104.2°) and had few defects in the crystalline arrays. The reconstituted Fourier map of the ice‐embedded crystal showed that there are two high contrast parts in one unit cell.