Premium
mRNA amplification system by viral replicase in transgenic plants
Author(s) -
Mod Masashi,
Kaido Masanori,
Okuno Tetsuro,
Furusawa Iwao
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81634-c
Subject(s) - brome mosaic virus , subgenomic mrna , cauliflower mosaic virus , rna dependent rna polymerase , biology , virology , rna , transgene , mosaic virus , microbiology and biotechnology , gene , plant virus , viral replication , genetically modified crops , virus , genetics
We have constructed transgenic tobacco plants (M1×2‐FCP2IFN plants) expressing viral RNA replication genes of brome mosaic virus (BMV) and BMV RNA3 derivative (FCP2IFN) carrying the human gamma Interferon (IFN‐γ) gene. In M1×2‐FCP2IFN plants the RNA3 derivative expressed from the integrated cDNA was replicated and subgenomic RNA (i.e. mRNA of IFN‐γ) was produced by BMV replicase. The accumulation level of the mRNA of IFN‐γ was approximately 5‐fold higher than that by the cauliflower mosaic virus (CaMV) 35S RNA promoter. In addition IFN‐γ accumulated in M1×2‐FCP2IFN plants.