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Region‐specific expression of multiple lactase‐phlorizin hydrolase genes in intestine of rabbit
Author(s) -
Villa Marco,
Brunschwiler Django,
Gächter Thomas,
Boll Werner,
Semenza Giorgio,
Mantei Ned
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81611-3
Subject(s) - biology , gene , lactase , microbiology and biotechnology , exon , small intestine , complementary dna , gene expression , messenger rna , hydrolase , coding region , genetics , biochemistry , enzyme
We have identified a total of 4 sequences coding for lactase‐phlorizin hydrolase (LPH) in the rabbit. Individual rabbits each yielded three different LPH cDNA sequences, or three chromosomal segments containing exon 1 of the LPH gene, representing either three genes or allelic variants of two genes. The three sequences were found in differing proportions in intestinal mRNA depending on the position along the small intestine from which the RNA was isolated. If all these mRNAs are translated, posttranslational mechanisms previously implicated in the regulation of LPH in the rabbit will be acting on different enzyme species in different parts of the intestine. However, we find no evidence for more than one LPH gene in the rat, and have previously shown that humans have only one LPH gene. p]Lactase‐phlorizin hydrolase; Developmental regulation; Small intestine; Gene family