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Relative stability of AT and GC pairs in parallel DNA duplex formed by a natural sequence
Author(s) -
Borisova O.F.,
Shchyolkina A.K.,
Chernov B.K.,
Tchurikov N.A.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81591-m
Subject(s) - acriflavine , dna , sequence (biology) , duplex (building) , chemistry , thermal stability , denaturation (fissile materials) , nucleic acid denaturation , fluorescence , random hexamer , nucleotide , gc content , crystallography , base sequence , biochemistry , organic chemistry , nuclear chemistry , physics , quantum mechanics , gene , genome
The low‐cooperative melting of parallel DNA formed by a natural 40 bp long sequence from Drosophila : 5′‐d(TGATTGATCGATTGTTTGCATGCACACGGTGAGCG)‐3′ 5′‐d(ACTAACTAGCTAACAAACGTACGTGTGCCACTCGC)‐3′ that possesses a normal nucleotide content was studied by using the special method of measuring the fluorescence of its complex with acriflavine as well as by conventional thermal denaturation. Acriflavine allows discrimination of the melting of AT and GC pairs because its fluorescence is quenched by neighbouring G bases. We have observed that about 40% of AT pairs melt at 14°C while the remainder melt at 42°C. The GC pairs remain stable up to ∼ 40°C and melt at 54°C. The higher stability of GC pairs suggests the formation of cis Watson‐Crick pairs in parallel DNA.