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A 31 P NMR study of the interaction of the antitumor active metallocene Cp 2 MoCl 2 with calf thymus DNA
Author(s) -
Harding Margaret M.,
Harden Grahame J.,
Field Leslie D.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81588-q
Subject(s) - metallocene , dna , chemistry , adduct , covalent bond , nuclear magnetic resonance spectroscopy , phosphate , stereochemistry , crystallography , organic chemistry , biochemistry , polymerization , polymer
Treatment of sonicated calf thymus DNA with the antitumor active metallocene Cp 2 MoCl 2 afforded a metallocene‐DNA complex which was characterized by 31 P NMR spectroscopy. In addition to the resonance for the phosphate backbone (δ −1.6), the spectrum contained 2 signals assigned to a phosphate bound Mo—DNA complex(es) (δ 37.2, 36.5) and a broad signal at δ 6.2 ppm. This result suggests that covalent attachment of the metallocene Cp 2 MoCl 2 occurs via phosphate(O) coordination and is accompanied by local distortion of the DNA backbone. This result supports recent ICP studies with Cp 2 TiCl 2 that have DNA detected DNA—metallocene adducts.