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High resolution proton NMR investigations of rat blood plasma Assignment of resonances for the molecularly mobile carbohydrate side‐chains of ‘acute‐phase’ glycoproteins
Author(s) -
Grootveld Martin,
Claxson Andrew W.D.,
Chander Chaman L.,
Haycock Peter,
Blake David R.,
Hawkes Geoffrey E.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81584-m
Subject(s) - glycoprotein , side chain , chemistry , carbohydrate , phase (matter) , nuclear magnetic resonance , biochemistry , organic chemistry , polymer , physics
An intense broad resonance at 2.14 ppm present in high field (400, 500 and 600 MHz) Hahn spin‐echo 1 H‐NMR spectra of rat blood plasma, but absent from those of human blood plasma is attributable to the presence of terminal O ‐acetylsialate sugars in the molecularly mobile carbohydrate side‐chains of ‘acute‐phase’ glycoproteins (predominantly α 1 ‐acid glycoprotein). The presence of such alternative acetylsugars in the carbohydrate side‐chains of rat plasma glycoproteins are of much physiological and experimental significance in view of the regular use of these animals in model systems of human inflammatory conditions.