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A 19 F NMR study of the interaction of 3‐fluoro‐4‐demethoxydaunomycin with the hexanucleotide d(TCCGGA) 2
Author(s) -
Barr Kathryn E.,
Russell Richard A.,
Warrener Ronald N.,
Collins J.Grant
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81562-e
Subject(s) - anthracycline , chemistry , footprinting , stereochemistry , nuclear magnetic resonance spectroscopy , dna , fluorine 19 nmr , crystallography , biochemistry , biology , base sequence , genetics , cancer , breast cancer
19 F NMR spectroscopy has been used to study the binding of the fluorinated anthracycline 3‐fluoro‐4‐demethoxydaunomycin with the hexanucleotide d(TCCGGA) 2 . In the spectrum of the 1:1 anthracycline‐d(TCCGGA) 2 complex four resonances of approximately equal intensity were observed. This indicated that 4‐demethoxydaunomycin intercalated at all possible sites with similar affinity. This suggests that the specific high affinity binding sites that are observed in anthracycline—DNA footprinting experiments are strongly regulated by the local DNA conformation.