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cDNA cloning of a new type of subunit of mammalian proteasomes
Author(s) -
Thomson Stuart,
Balson Deborah F.,
Rivett A.Jennifer
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81553-c
Subject(s) - complementary dna , protein subunit , proteasome , peptide sequence , protein primary structure , cloning (programming) , biology , amino acid , microbiology and biotechnology , nucleic acid sequence , cdna library , biochemistry , gene , computer science , programming language
The primary structure of a new type of subunit (RN3) of rat proteasomes (multicatalytic proteinase complexes) has been determined from the nucleotide sequence of the cDNA. The cDNA encodes a protein of 232 amino acids but the directly determined N‐terminal amino acid sequence suggests that the subunit is post‐translationally processed to a M r = 24k form. Sequence alignments reveal a similarity of RN3 to other proteasome subunits. It can be designated a B‐type proteasomal subunit but is not closely related to the β subunit of the archaebacterial proteinase or to other members of the B group.