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Two different promoters direct expression of two distinct forms of mRNAs of human platelet‐activating factor receptor
Author(s) -
Mutoh Hiroyuki,
Bito Haruhiko,
Minami Michiko,
Nakamura Motonao,
Honda Zenichiro,
Izumi Takashi,
Nakata Ryo,
Kurachi Yoshihisa,
Terano Akira,
Shimizu Takao
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81552-b
Subject(s) - promoter , microbiology and biotechnology , platelet activating factor receptor , receptor , chemistry , biology , gene expression , genetics , gene , antagonist
The human platelet‐activating factor (PAF) receptor gene exists as a single copy on chromosome 1. We identified two 5′‐noncoding exons, each of which has distinct transcriptional initiation sites. These exons are alternatively spliced to a common splice acceptor site on a third exon that contains the total open reading frame to yield two different species of functional mRNA (Transcript 1 and 2). Transcript 1 has consensus sequences for transcription factor NF‐κB and Sp‐1, and the Initiator ( Inr ) sequence homologous to the murine terminal deoxynucleotidyltransferase gene. Transcript 2 also contains consensus sequences for transcription factor AP‐1, AP‐2, and Sp‐1. Transcripts 1 and 2 were both detected in heart, lung, spleen, and kidney, whereas only Transcript 1 was found in peripheral leukocytes, a differentiated human eosinophilic cell line (EoL‐1 cells), and brain. Existence of distinct promoters was thus suggested to play a role in the regulatory control of PAF receptor gene expression in different human tissues and cells.