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A brain‐specific transcript from the 3′‐terminal region of the skeletal muscle ryanodine receptor gene
Author(s) -
Takeshima Hiroshi,
Nishimura Seiichiro,
Nishi Miyuki,
Ikeda Michiko,
Sugimoto Tetsuo
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81547-d
Subject(s) - ryanodine receptor , skeletal muscle , biology , complementary dna , microbiology and biotechnology , endoplasmic reticulum , cardiac muscle , gene , biochemistry , endocrinology
We have shown previously that the skeletal muscle ryanodine receptor mRNA of ∼16,000 nucleotides codes 5,037 amino acid residues constituting the calcium release channel in skeletal muscle. In this study, RNA blot hybridization analysis shows that the brain contains an RNA species with an estimated size of ∼2,400 nucleotides hybridizable with the 3′‐terminal region of the skeletal muscle ryanodine receptor cDNA. cDNA cloning and genome analysis indicated that two transcripts differing in their start sites are produced from the skeletal muscle ryanodine receptor gene in a tissue‐specific fashion, and that the mRNA in brain may code the carboxyl‐terminal region of the ryanodine receptor molecule. cDNA expression experiments suggested that the ATG triplet encoding Met 4382 of the skeletal muscle ryanodine receptor can function as a translation initiation codon, and that the expressed protein composed of the carboxy terminal 656 amino acid residues of the receptor is located on the endoplasmic reticulum membrane.