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Expression of PP2A B regulatory subunit β isotype in rat testis
Author(s) -
Hatano Yoshiaki,
Shima Hiroshi,
Haneji Tatsuji,
Miura Akira B.,
Sugimura Takashi,
Nagao Minako
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81535-8
Subject(s) - spermatid , protein phosphatase 2 , microbiology and biotechnology , biology , complementary dna , messenger rna , beta (programming language) , isotype , protein subunit , phosphatase , amino acid , alpha (finance) , spermatogenesis , gene , genetics , phosphorylation , endocrinology , antibody , medicine , construct validity , nursing , patient satisfaction , computer science , monoclonal antibody , programming language
We isolated a rat cDNA encoding part of the β‐isotype of the B regulatory subunit (BRß) of protein phosphatase 2A (PP2A). The isolated cDNA encoded the region corresponding to amino acids positions 8(R) to 177(N) of human BRß. The identities of the nucleotide and amino acid sequences of the rat and human BRßs were 95.7% and 100%, respectively. The BR ß mRNA was specifically expressed in rat brain and testis, the lengths of mRNAs in these two organs being different. In the testis, the BRß mRNA was first detected 40 days after birth, increasing gradually thereafter, and was expressed specifically in elongated spermatids, while mRNA of the α‐isotype (BRα) was expressed equally in all spermatogenic cells. After meiosis, round spermatids change morphologically to elongated spermatids. BRß may regulate the activity of the PP2A catalytic subunit in spermatids, and be involved in spermatogenic maturation, especially spermatid elongation.