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Calvasculin, as a factor affecting the microfilament assemblies in rat fibroblasts transfected by src gene
Author(s) -
Watanabe Yasuo,
Usada Nobuteru,
Minami Hiroyuki,
Morita Takashi,
Tsugane Shinichiro,
Ishikawa Ryoki,
Kohama Kazuhiro,
Tomida Yasuhiro,
Hidaka Hiroyoshi
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81530-d
Subject(s) - microfilament , transfection , actin , microbiology and biotechnology , cytoskeleton , cell , biology , transformation (genetics) , messenger rna , gene , chemistry , genetics
Cell transformations accompany alterations in cell morphology and microfilament patterns. Calvasculin encodes mRNA termed pEL‐98, 18A2, 42A, p9Ka, or mtsl, found to be elevated in several metastatic cell lines. We report the elevation of calvasculin expression in SR‐3Y1 cells, which show disappearance of ordered microfilaments, compared to that in 3Y1 cells and that the similar distribution of calvasculin to that of actin filaments. Interestingly, calvasculin co‐sediments with F‐actin and bundles actin filaments in a Ca 2+ ‐dependent manner. This activity, along with the elevation of calvasculin following transformation, suggests that the disorganization of filaments in SR‐3Y1 cell is due to the cross‐linking activity of calvasculin.