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A structural model for the glutamate‐specific endopeptidase from Streptomyces griseus that explains substrate specificity
Author(s) -
Barbosa João Alexandre R.G.,
Garratt Richard C.,
Saldanha José W.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81529-9
Subject(s) - streptomyces griseus , endopeptidase , proteases , serine , biochemistry , enzyme , stereochemistry , histidine , chemistry , substrate (aquarium) , biology , streptomyces , bacteria , genetics , ecology
We present a model for the three‐dimensional structure of the glutamate‐specific endopeptidase from Streptomyces griseus based on the crystal structures of other bacterial proteases of the trypsin family. For the first time a structural model is described which attempts to explain the basis of P1 glutamate specificity in serine proteases. Several important changes to the S1 pocket with respect to other members of the family of different specificity are described. Of particular interest is the presence of a histidine at position 213 and the substitution of Arg‐138 by lysine. Other biochemical evidence concerning substrate preferences can be rationalized on the basis of the model.