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Isolation and characterization of PSII core complexes from a brown alga, Laminaria saccharina
Author(s) -
Douady Dominique,
Rousseau Bernard,
Berkaloff Claire
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81524-4
Subject(s) - fucoxanthin , laminaria , chemistry , laminaria digitata , pheophytin , saccharina , chlorophyll , chloroplast , pigment , biochemistry , botany , biology , photosystem ii , algae , photosynthesis , organic chemistry , gene
PSII‐enriched particles, active for DCIP‐reduction, were prepared from Laminaria saccharina chloroplasts, and PSII core complexes were further purified by ion‐exchange chromatography. They contained several polypeptides, four of them cross‐reacting with antibodies raised against CP47, CP43, D1 and D2 of green plants. A second chromatography was required to separate: (i) a core antenna, composed of 51 kDa polypeptide subunits, binding 11 β‐carotene, 4 chlorophyll (Chl) c and 7 fucoxanthin for 100 Chl a , and reacting with CP47 antibodies; and (ii) a reaction center complex consisting of two main polypeptides of 34 and 36 kDa. The pigment stoichiometry was of 5 Chl a and 0.5 β‐carotene for 2 pheophytin a . The 34 and 36 kDa components cross‐reacted with anti‐D1 and anti‐D2 antibodies, respectively. The presence of cytochrome b ‐559 was substantiated by spectrophotometry.