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A novel human tyrosine kinase gene inducible in T cells by interleukin 2
Author(s) -
Tanaka Nobuyuki,
Asao Hironobu,
Ohtani Kiyoshi,
Nakamura Masataka,
Sugamura Kazuo
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81520-a
Subject(s) - sh2 domain , proto oncogene tyrosine protein kinase src , microbiology and biotechnology , tyrosine kinase , tyrosine , gene , biology , sh3 domain , subfamily , protein kinase domain , tyrosine phosphorylation , biochemistry , kinase , signal transduction , mutant
We have cloned a novel human protein tyrosine kinase gene specific to T cells by the polymerase chain reaction method. This gene encodes a 620 amino‐acid polypeptide including a catalytic domain for tyrosine kinase, an SH2 domain and an SH3 domain, seemingly belonging to the src family. However, characteristics of a long unique N‐terminal stretch and lack of a myristylation site at the N‐terminus and of a kinase regulatory tyrosine residue in the C‐terminus classify this molecule into a new subfamily comprising recently cloned mouse tec, itk/tsk and human atk/bpk genes. This gene was transcriptionally induced in normal T cells by interleukin 2 stimulation. These results suggest the crucial roles of this gene in T cell proliferation and differentiation.