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Two 3',5'‐cyclic‐adenosine monophosphate response elements in the promoter region of the human gastric inhibitory polypeptide gene
Author(s) -
Someya Yoshimichi,
Inagaki Nobuya,
Maekawa Toshio,
Seino Yutaka,
Ishii Shunsuke
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81493-j
Subject(s) - inhibitory postsynaptic potential , gene , adenosine , cyclic adenosine monophosphate , chemistry , biochemistry , microbiology and biotechnology , biology , endocrinology , receptor
Transfection of chimeric chloramphenicol acetyltransferase plasmids containing various deletions of the human gastric inhibitory polypeptide (GIP) promoter into hamster insulinoma (HIT T15) cells indicated that the region between −180 and +14 is sufficient for basal promoter activity. Two CRE‐BP1 binding sites were identified in this promoter region by DNase I footprinting with the bacterially expressed cAMP response element (CRE) binding protein, CRE‐BP1. Mutation analyses showed that these two CREs are required for the basal promoter activity, and furthermore that one site, at nucleotide‐158, contributed mainly to the cAMP inducibility of the GIP promoter in HIT T15 cells. Interestingly, the GIP promoter activity was repressed by the c‐ jun proto‐oncogene product, possibly through the CREs.