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Induction of metallothionein in a human astrocytoma cell line by interleukin‐1 and heavy metals
Author(s) -
Kikuchi Yutaka,
Irie Masachika,
Kasahara Tadashi,
Sawada Jun-ichi,
Terao Tadao
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81483-g
Subject(s) - metallothionein , cytoplasm , intracellular , immunofluorescence , cell culture , monoclonal antibody , microbiology and biotechnology , chemistry , nucleus , biology , antibody , biochemistry , immunology , gene , genetics
The effects of cytokines and heavy metals on the expression and localization of metallothioneins (MTs) within U373MG astrocytoma cells were analyzed by using indirect immunofluorescence using a monoclonal anti‐MT antibody (MT45). IL‐1, CdCl 2 (50μM) or ZnCI 2 , (500μM) remarkably augmented intracellular MT levels, whereas IL‐6 or 10 μM of ZnCl 2 showed no inducing activity. From 24 to 48 h after the addition of CdCl 2 or IL‐1, immunoreactive MTs were found in the cytoplasm and the nucleus. After 72 h, immunoreactive MTs accumulated in a granular form near the cell surfaces in the presence of CdCl 2 (50 μM) or IL‐1 plus ZnCI 2 (10 μM). However, this accumulation was not observed when only IL‐1 was added. Thus, Zn 2+ facilitated the appearance of the granular form of immunoreactive MTs at a concentration where they do not induce MTs by themselves.