Premium
Glycosaminoglycans exposed on the endothelial cell surface
Author(s) -
Cavari Simone,
Vannucchi Simonetta
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81469-g
Subject(s) - dermatan sulfate , glycosaminoglycan , heparin , heparan sulfate , chondroitin sulfate , endothelial stem cell , chemistry , sulfation , chondroitin , biochemistry , in vitro , cell , endothelium , biology , endocrinology
We have analyzed the glycosaminoglycans exposed on the surface of endothelial cells cultured in vitro. The cells were labelled with [ 35 S]sodium sulfate. Chondroitin sulfate, heparan sulfate and dermatan sulfate are the main sulfated glycosaminoglycans present on the cell surface. They are synthesized by endothelial cells. However, the electrophoretic analysis and corresponding autoradiography of the glycosaminoglycans removed from the endothelial cell surface shows the presence of heparin‐like compound which is not synthesized by the cells as it is unlabelled by [ 35 S]sodium sulfate. We show here that a proteolytic treatment of the commonly used serum for cell culturing, reveals the presence of an heparin‐like anticoagulant activity. These results suggest that the endothelial cells bind endogenous heparin‐like molecules present in serum.