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Saccharomyces cerevisiae multifunctional protein RAP1 binds to a conserved sequence in the Polyoma virus enhancer and is responsible for its transcriptional activity in yeast cells
Author(s) -
Pollice Alessandra,
Ciaramella Maria,
Pulitzer John F.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81452-6
Subject(s) - enhancer , saccharomyces cerevisiae , yeast , transcription (linguistics) , biology , rap1 , binding site , microbiology and biotechnology , binding domain , transcription factor , biochemistry , gene , linguistics , philosophy
The Polyoma virus enhancer (A + B domain) activates transcription in Saccharomyces cerevisiae when joined to appropriate yeast promoter elements. We demonstrate by DNase I footprints and inhibition of binding by specific antibody, that the yeast protein RAP1 binds to the B‐domain of the Polyoma enhancer and, at least in some promoter contexts, is responsible for transcriptional activity of the enhancer B‐domain in yeast. Close matches to a consensus RAP1‐binding site are also present in other viral enhancers.