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Detection of minor adducts in cisplatin‐modified DNA by transcription footprinting
Author(s) -
Decoville Martine,
Schwartz Annie,
Locker Daniel,
Leng Marc
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81447-8
Subject(s) - dna , adduct , chemistry , insert (composites) , dna footprinting , transcription (linguistics) , footprinting , polymerase , microbiology and biotechnology , base pair , rna , stereochemistry , biology , biochemistry , gene , dna binding protein , transcription factor , base sequence , organic chemistry , linguistics , philosophy , mechanical engineering , engineering
Two DNA restriction fragments containing either a d(GC) 5 or a d(TTGCTTGATTAGTTGTGTT) insert were subjected to reaction with cis ‐diamminedichloroplatinum(II) and were then used as templates for RNA synthesis by T7 RNA polymerase. Within the d(GC) 5 insert, interstrand cross‐links are preferentially formed. Within the second insert, the reactivity order of the potential binding sites is d(ApG) > d(GpC/ GpC) = d(GpA) > d(GpTpG). In the presence of cyanide ions, the adducts are much less stable at the d(GpA) sites than at the d(GpCpG) sites, in double‐stranded DNA.