Premium
Occurrence and sequence of a DnaJ protein in plant ( Allium porrum ) epidermal cells
Author(s) -
Bessoule J.-J.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81446-7
Subject(s) - biology , complementary dna , open reading frame , microbiology and biotechnology , clone (java method) , cdna library , molecular cloning , allium , gene , peptide sequence , biochemistry , botany
Antibodies raised against a purified fraction from microsomal membranes of leek epidermal cells were used to screen a λzap expression library from epidermal cells of leek plants. A near full‐length clone was isolated. This cDNA contains an open reading‐frame of 1,191 bp coding for a DnaJ protein (leek DNAJ 1 or LDJ1). Leek DnaJ1 represents the second protein of this type described in a pluricellular organism, the first being that sequenced from human cells.