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Location of tolerated insertions/deletions in the structure of the maltose binding protein
Author(s) -
Betton Jean-Michel,
Martineau Pierre,
Saurin William,
Hofnung Maurice
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81409-s
Subject(s) - maltose , maltose binding protein , chemistry , protein structure , computational biology , biochemistry , biophysics , genetics , biology , gene , recombinant dna , fusion protein , enzyme
In a previous study [(1987) J. Mol. Biol. 194, 663‐673], we isolated ten insertion/deletion mutants (indels) of the maltose binding protein for which the maltose binding constant was only a little or not at all affected. In this paper, we have localized these mutations in the recently solved three‐dimensional structure. Contrary to the general expectation, most of the insertion/deletion modifications occurred within elements of secondary structure. An analysis of the inserted residues for three indels found within α helices allowed an interpretation regarding protein structure accommodation to such modifications.