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Correlation between phospholipase A 2 activity and intra‐Golgi protein transport reconstituted in a cell‐free system
Author(s) -
Tagaya Mitsuo,
Henomatsu Nobuhiro,
Yoshimori Tamotsu,
Yamamoto Akitsugu,
Tashiro Yutaka,
Fukui Toshio
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81393-e
Subject(s) - phospholipase a2 , golgi apparatus , phospholipase , biochemistry , chemistry , secretion , transport protein , phospholipase a , enzyme , cell , biology
A wide variety of phospholipase A 2 inhibitors blocks intra‐Golgi protein transport reconstituted in a cell‐free system. Phospholipase A 2 activity detectable under the protein transport assay conditions is actually inhibited by the inhibitors. There is a good correlation between the inhibition of protein transport and that of phospholipase A 2 activity. Prolactin secretion from GH 3 cells is also blocked by a membrane‐permeable phospholipase A 2 , inhibitor, suggesting the physiological relevance to inhibition of protein transport in vitro by phospholipase A 2 inhibitors.