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A product of phosphatidylinositol‐3 kinase is elevated in dividing HT29 colonic epithelial cells
Author(s) -
Dobos Gustav J.,
Wu Xiao Rong,
Traynor-Kaplan Alexis
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81381-9
Subject(s) - phosphatidylinositol , mitosis , kinase , pi , cell division , phospholipid , cell growth , microbiology and biotechnology , biology , biochemistry , cell cycle , cell culture , cell , chemistry , genetics , membrane
Phosphatidylinositol 3 kinase (PI‐3 kinase) activity has been linked to cell proliferation and growth regulation. Therefore, we studied changes in phosphoinositide metabolism during the cell cycle of HT‐29 cells, a colonic epithelial cell line. HT29 cells were treated with the microtubule disrupter, nocodozole, separated into mitotic and quiescent populations and their phospholipid composition was analyzed. Radiolabelled phospholipids from cells labelled with 32 PO 4 or [ 3 H]myoinositol were analyzed by TLC and/or deacylated and analyzed by HPLC. In all cases, levels of phosphatidyl‐inositol 3 phosphate from mitotic phase cells were double that in resting populations. Therefore, levels of a product of PI‐3 kinase are elevated and may play a role in cell division.