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Recombinant GABA A receptor function and ethanol
Author(s) -
Sigel Erwin,
Baur Roland,
Malherbe Pari
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81380-i
Subject(s) - xenopus , gabaa receptor , protein subunit , ethanol , chemistry , stimulation , receptor , splice , recombinant dna , biophysics , biochemistry , microbiology and biotechnology , biology , endocrinology , gene
Different combinations of cloned subunits of the rat brain GABA A receptor were expressed in Xenopus oocytes. Possible effects of ethanol on the expressed GABA‐induced chloride current were determined. The consequence of replacing the γ 2s subunit by the alternatively spliced variant γ 2L was specifically tested on the responsiveness to ethanol. A significant stimulation of the GABA response was only observed at very high concentrations (> 60 mM) of ethanol. No differential response was observed between subunit combinations containing different γ 2 subunit splice variants.