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Molecular cloning of a human β3‐adrenergic receptor cDNA
Author(s) -
Lelias J.M.,
Kaghad M.,
Rodriguez M.,
Chalon P.,
Bonnin J.,
Dupre I.,
Delpech B.,
Bensaid M.,
LeFur G.,
Ferrara P.,
Caput D.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81377-c
Subject(s) - complementary dna , exon , microbiology and biotechnology , gene , biology , molecular cloning , cloning (programming) , cdna library , intron , rapid amplification of cdna ends , peptide sequence , beta (programming language) , genetics , computer science , programming language
We report the molecular cloning of a β3‐adrenergic receptor β3‐AR cDNA from human brown adipose tissue. The cDNA‐encoded protein is identical to the previously cloned β3‐AR but with 6 additional amino acids at the C‐terminus. The C‐terminus is shared by the β3 receptors expressed in human neuroblastoma cells [SK‐N‐MC] [Mol. Pharmacol. 42 (1992) 964‐970]. Furthermore, using a polymerase chain reaction strategy we have cloned and sequenced the β3‐AR introns. Sequence analysis demonstrates that the human β3‐AR gene comprises at least 3 exons and 2 introns and that the most abundant β3‐AR transcripts encode a protein with an exon 3‐derived C‐terminus. Interestingly, although a similar organization has been found in rodent genes, the rat β3‐AR transcripts encode a receptor with an exon 2‐derived C‐terminus.