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Calcium‐induced degradation of the Inositol (1,4,5)‐trisphosphate receptor/Ca 2+ ‐channel
Author(s) -
Magnusson Andres,
Haug Lise Sofie,
Walaas S.Ivar,
Østvold Anne Carme
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81345-z
Subject(s) - receptor , inositol , inositol trisphosphate receptor , cytosol , inositol phosphate , chemistry , intracellular , calcium , biophysics , biochemistry , proteolysis , microbiology and biotechnology , biology , enzyme , organic chemistry
Ca 2+ ‐induced degradation of the neuronal inositol (1,4,5)‐trisphosphate receptor, a protein which regulates Ca 2+ ‐release from intracellular stores, has been examined. The IP 3 ‐receptor, immunopurified from rat cerebellum, appeared to be an excellent substrate for purified Ca 2+ ‐activated neutral protease (calpain). Incubation of membranes or immunopurified IP 3 ‐receptor with Ca 2+ and cerebellar cytosol also resulted in degradation of the receptor. Two main fragments with approximate molecular masses of 130 and 95 kDa were generated, both of which appeared to derive from the carboxyterminal Ca 2+ ‐channel‐containing part of the protein. These data suggest that activation of the IP 3 ‐receptor, by causing increases in intracellular [Ca 2+ ], might result in degradation of the N‐terminal, IP 3 ‐binding part of the receptor.