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Tumour necrosis factor‐α increases the ubiquitinization of rat skeletal muscle proteins
Author(s) -
García-Martínez Cèlia,
Agell Neus,
Llovera Marta,
López-Soriano Francisco J.,
Argilés Josep M.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81341-v
Subject(s) - skeletal muscle , tumor necrosis factor alpha , ubiquitin , cytokine , endocrinology , soleus muscle , proteolysis , medicine , sepsis , protein degradation , necrosis , inflammation , myocyte , chemistry , recombinant dna , biology , biochemistry , enzyme , gene
An acute intravenous administration of 100 μg/kg body weight of recombinant tumour necrosis factor‐α (TNF) resulted in a time‐dependent increase in the levels of both free and conjugated ubiquitin in rat skeletal muscle. The effects of the cytokine were more pronounced in the red muscle soleus than in the white muscle EDL. In the former muscle type, TNF‐treatment also resulted in a time‐dependent increase in the percentage of free ubiquitin. The results suggest that the ubiquitin system for non‐lysosomal protein degradation could have a very important role in the mechanism triggered by TNF which is responsible for enhanced muscle proteolysis in sepsis and other pathological states.