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Around the growth phase transition S. cerevisiae's make‐up favours sustained oscillations of intracellular metabolites
Author(s) -
Richard Peter,
Teusink Bas,
Westerhoff Hans V.,
van Dam Karel
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81332-t
Subject(s) - intracellular , nad+ kinase , intracellular ph , glycolysis , biochemistry , extracellular , yeast , chemistry , metabolism , substrate (aquarium) , biophysics , biology , enzyme , ecology
Under a limited set of hitherto incompletely defined conditions, inhibition of respiration has been shown to cause transient oscillations in NAD(P)H fluorescence of yeast cells. In this paper, we apply a new method [1992, Anal. Biochem. 204, 118‐132] for extraction of intracellular metabolites. This method involves spraying the cells into −40°C methanol; the neutral pH allows extraction of nearly all intracellular metabolites, including NADH. Close to the shift from glucose to ethanol as a growth substrate, the cells acquire a make‐up amenable to sustained oscillations in intracellular concentrations of NADH and glycolytic intermediates such as glucose‐6‐phosphate. NADH was found to oscillate between 200 μM and 400 μM intracellular concentration. The cellular make‐up determining the tendency to oscillate is ‘remembered’ by the cells after three hours of starvation.