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Glutathione in whole blood: a novel determination using double quantum coherence transfer proton NMR spectroscopy
Author(s) -
Mason Ralph P.,
Cha Gloria H.,
Gorrie George H.,
Babcock Evelyn E.,
Antich Peter P.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81321-p
Subject(s) - spectroscopy , glutathione , coherence (philosophical gambling strategy) , nuclear magnetic resonance spectroscopy , whole blood , chemistry , proton , proton nmr , analytical chemistry (journal) , nuclear magnetic resonance , chromatography , biochemistry , physics , stereochemistry , biology , nuclear physics , enzyme , quantum mechanics , immunology
Double quantum selective coherence transfer proton NMR spectroscopy has been used to observe glutathione in whole blood. The efficient water suppression of this technique avoids the need to resuspend the cells in D 2 O, hence avoiding equilibrium and kinetic isotope effects. Using this method we estimate the concentration of glutathione in fresh whole rabbit blood at ~1.7 mM.