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A novel nucleosome assembly procedure (with a little help from pectin)
Author(s) -
Sobolewski Christopher H.M.,
Klump Horst H.,
Lindsey George G.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81320-y
Subject(s) - polyglutamic acid , pectin , depolymerization , chemistry , polysaccharide , biochemistry , pectin lyase , pectinase , polymer chemistry , enzyme
The acidic polysaccharide pectin (α‐1,4‐polygalacturonic acid) has been introduced as a nucleosome assembly facilitator as a substitute for polyglutamic acid. The pectin‐assembled nucleosomes were indistinguishable from polyglutamic acid‐assembled nucleosomes by thermal denaturation and DNAse I digestion. Pectin had two major advantages over polyglutamic acid ‐ the yield of assembled cores was approximately 50% higher and the pectin could be easily removed after completion of the reassembly procedure by dialysis following pectinase cleavage.