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Molecular cloning of a novel rat G‐protein‐coupled receptor gene expressed prominently in lung, adrenal, and liver
Author(s) -
Harrison Jeffrey K.,
Barber Cynthia M.,
Lynch Kevin R.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81318-t
Subject(s) - biology , complementary dna , microbiology and biotechnology , open reading frame , messenger rna , gene , cdna library , genomic library , rapid amplification of cdna ends , molecular cloning , gene expression , peptide sequence , genetics
A gene and cDNA (named G10d) encoding a novel member of the G‐protein‐coupled receptor (GCR) superfamily has been isolated from a rat genomic library and a rat liver cDNA library by tandom polymerase chain reaction (PCR) amplification and hybridization screening. Comparison of the sequence of the encoded protein to other members of the rhodopsin family of the GCR superfamily suggests that this new member is a peptide receptor. The G10d gene is present as a single copy in the rat genome and its translational open reading frame is intronless. Northern analysis demonstrates a wide and uneven distribution of G10d mRNA in the adult rat, with the highest mRNA abundance in lung, liver and adrenal gland extracts.