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Detection of a plant protein analogous to the yeast spliceosomal protein, PRP8
Author(s) -
Kulesza Helen,
Simpson Gordon G.,
Waugh Robbie,
Beggs Jean D.,
Brown John W.S.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81315-q
Subject(s) - snrnp , nicotiana tabacum , yeast , rna splicing , fusion protein , spliceosome , biology , saccharomyces cerevisiae , epitope , biochemistry , microbiology and biotechnology , genetics , antibody , gene , rna , recombinant dna
We have investigated whether a splieeosomal protein analogous to the yeast protein, PRP8, was present in higher plants. A protein with a molecular weight > 200 kDa was detected in Western blots of tobacco ( Nicotiana tabacum L.) nuclear extracts with affinity‐purified antibodies, raised against four different β‐galactosidase‐PRP8 fusion proteins. The > 200 kDa protein was also immunoprecipitated by antibodies against the snRNA‐specific trimethylguanosine cap structure and was, therefore, snRNP‐associated. The presence of this protein in plants, in addition to yeast, Drosophila and humans, and the conservation of large size and epitopes highlights the importance of PRP8 in pre‐mRNA splicing.