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Forskolin stimulates porcine sperm capacitation by increasing calcium uptake
Author(s) -
Okamura Naomichi,
Tanba Michiko,
Fukuda Atsunori,
Sugita Yoshiki,
Nagai Taku
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81309-n
Subject(s) - forskolin , capacitation , acrosome reaction , verapamil , sperm , calcium , calcium in biology , chemistry , endocrinology , medicine , andrology , microbiology and biotechnology , biology , stimulation
Using the fluorescent calcium indicator fura‐2, forskolin was found to dose‐dependently cause an immediate increase in the concentration of intracellular free calcium of porcine cauda epididymal sperm. This stimulatory effect of forskolin is due to the enhancement of Ca 2+ uptake by the verapamil‐sensitive transporter on the sperm plasma membrane and results in the promotion of the sperm capacitation and subsequent acrosome reaction.