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Mosquito DNA polymerase ε
Author(s) -
Lee Seung-Koo,
Fuchs Morton S.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81304-i
Subject(s) - dna polymerase , dna clamp , dna polymerase ii , polymerase , microbiology and biotechnology , proliferating cell nuclear antigen , biology , dna polymerase i , exonuclease , dna polymerase delta , klenow fragment , biochemistry , dna , polymerase chain reaction , reverse transcriptase , gene
The mosquito homolog of mammalian DNA polymerase ϵ, formerly known as a proliferating cell nuclear antigen (PCNA)‐independent form of DNA polymerase δ, has been purified from mosquito larval extracts. The polymerase ϵ was separated from DNA polymerase α by chromatography on hydroxylapatite, and the enzyme was subsequently purified on single‐stranded DNA agarose, followed by a 5' AMP‐agarose chromatography step. The purified polymerase exhibits an intrinsic 3'‐5' exonuclease activity and shows high activity using an oligo‐primed DNA template. Neither human nor Drosophila PCNA stimulated this polymerase activity. Additional immunochemical and biochemical evidence indicates that this enzyme is distinct from DNA polymerase α.