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Effect of platelet activating factor on the kinetics of LDL oxidation in vitro
Author(s) -
Deigner Hans-Peter,
Dresel Hans Alois
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81276-6
Subject(s) - kinetics , in vitro , chemistry , platelet , platelet activation , biophysics , biochemistry , microbiology and biotechnology , medicine , biology , physics , quantum mechanics
Oxidatively modified low‐density‐lipoprotein (LDL) might contribute to the atherosclerotic process. This study was performed to examine an effect of platelet‐activating factor (PAF) and of synthetic PAF analogs on Cu(II) induced oxidation of LDL in vitro: The d ‐ and l ‐isomers of PAF and analogs with short‐chain sn ‐2‐substituents, 1‐ O ‐alkyl‐2‐butyryl‐ sn ‐glycero‐3‐phosphocholine and 1‐ O ‐alkyl‐2‐heptanoyl‐ sn ‐glycero‐3‐phosphocholine, were found to be the most effective inhibitors of LDL oxidation. Oxidation was inhibited completely at PAF concentrations above 100 μM. Lyso‐PAF and analogs carrying longer chains at the sn ‐2 position were less effective. These results thus provide evidence for the involvement of other parameters in LDL oxidation beyond the content of natural antioxidants like vitamin E and β‐carotene.